| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Skidmore, Holden D (12)
|
MDEST |
45.83 |
41.16 |
-4.67 |
| 2 |
Farls, Benjamin A (12)
|
MDLBA |
48.35 |
42.12 |
-6.23 |
| 3 |
Navarra, Niall J (12)
|
MDMAS |
46.81 |
42.23 |
-4.58 |
| 4 |
Kunkel, Charlie M (11)
|
MDLBA |
45.70 |
42.80 |
-2.90 |
| 5 |
Kegley, Shep M (12)
|
MDSPRC |
|
43.36 |
- |
| 6 |
Emerson, Barret H (11)
|
MDEST |
41.78 |
43.82 |
2.04 |
| 7 |
Liang, Kai H (12)
|
MDEST |
49.44 |
43.89 |
-5.55 |
| 8 |
Smith, Charles W (11)
|
MDEST |
43.73 |
44.08 |
0.35 |
| 9 |
Fiori, James T (11)
|
MDMAS |
54.36 |
44.11 |
-10.25 |
| 10 |
Havens, Leo D (12)
|
MDSPRC |
48.51 |
44.56 |
-3.95 |
| 11 |
Peoples, Grayson B (12)
|
MDGBSA |
53.08 |
44.97 |
-8.11 |
| 12 |
Mitchell, Topher J (11)
|
MDLBA |
49.74 |
49.02 |
-0.72 |
| 13 |
Cui, Nicholas (12)
|
MDEST |
54.51 |
49.16 |
-5.35 |
| 14 |
Siri, Braden W (11)
|
MDMAS |
|
50.26 |
- |
| 15 |
Wright, Lucas J (12)
|
MDGBSA |
|
50.30 |
- |
| 16 |
Glisson, Keith (11)
|
MDGBSA |
52.21 |
51.62 |
-0.59 |
| 17 |
Asiedu, Eli E (12)
|
MDCSAC |
|
52.04 |
- |
| 18 |
Keiner, Andrew D (11)
|
MDEST |
55.65 |
52.85 |
-2.80 |
| 19 |
Mooney, Justin M (11)
|
MDLBA |
|
53.62 |
- |
| 20 |
Pustavoitau, Zachary A (11)
|
MDEST |
59.51 |
55.35 |
-4.16 |
| 21 |
Farah, Matthew J (11)
|
MDGBSA |
1:03.45 |
56.12 |
-7.33 |
| 22 |
Ackerman, William L (11)
|
MDSPY |
1:02.40 |
57.04 |
-5.36 |
| 23 |
Woldeselassie, David T (11)
|
MDEST |
1:00.42 |
58.68 |
-1.74 |
| 24 |
Ivey, Keith R (11)
|
MDSPRC |
|
1:00.93 |
- |
| 25 |
Merrill, Ian T (11)
|
MDHSST |
1:02.98 |
1:03.44 |
0.46 |
| 26 |
Luberski, Luke E (11)
|
MDBAAC |
|
1:27.36 |
- |
|
Anderson, Benjamin M (12)
|
MDSPRC |
|
NS |
- |
|
Di Gregorio, Caleb E (12)
|
MDMAS |
54.62 |
NS |
- |
|
Feracci, Dominic V (12)
|
MDLBA |
52.01 |
NS |
- |
|
Tetrault, Caden J (12)
|
MDSPRC |
|
NS |
- |
|
Celtnieks, Eli H (12)
|
MDLBA |
48.00 |
NS |
- |